Theses and Dissertations
This collection contains the theses and dissertations approved for public release from Texas A&M University.
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Liu, Lei (May 2009)[more][less]
Abstract: A variety of experiments were carried out to study the dispersion and microstructure of carbon nanotubes in aqueous suspensions and polymer composites with the goal to improve the electrical conductivity of the composites containing nanotubes. Epoxy composites containing covalently and noncovalently functionalized nanotubes were compared in terms of electrical and mechanical behavior. Covalent functionalization of nanotubes is based on chemical attachments of polyethylenimine (PEI) whereas noncovalent functionalization takes place through physical mixing of nanotubes and PEI. The electrical conductivity is reduced in composites containing covalently functionalized nanotubes due to damage of the tube?s conjugated surface that reduces intrinsic conductivity. Conversely, the mechanical properties are always better for epoxy composites containing covalently functionalized nanotubes. Clay particles were used as a rigid dispersing aid for nanotubes in aqueous suspensions and epoxy composites. When both nanotubes and clay were introduced into water by sonication, the suspension is stable for weeks, whereas the nanotubes precipitate almost instantly for the suspension without clay. In epoxy composites, nanotubes form separated clusters of aggregation, whereas a continuous threedimensional nanotube network is achieved when clay is introduced. Electrical conductivity of the epoxy composite is shown to significantly improve with a small addition of clay and the percolation threshold is simultaneously decreased (from 0.05 wt% nanotubes, when there is no clay, to 0.01 wt% when 2 wt% clay is introduced). The addition of clay can also improve the mechanical properties of the composites, especially at higher clay concentration. Weak polyelectrolytes (i.e., pH-responsive polymers) were also studied for their interaction with nanotubes and the electrical properties of the dried composite films. When dispersed by sonication, Nanotubes show pH-dependent dispersion and stability in poly(acrylic acid) water solution, as evidenced by changes in suspension viscosity and cryo-TEM images. The nanotube suspensions were then dried under ambient conditions and the composite films exhibit tailorable nanotube dispersion as a function of pH. The percolation threshold and maximum electrical conductivity are reduced when the pH is changed from low to high. Some other pH-responsive polymers were also studied, but their pH-dependent viscosity and conductivity were not as large or reversible as poly(acrylic acid). URI: http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-520 Files in this item: 1
Liu, Lei.pdf (7.287Mb) -
Joung, Young (December 2008)[more][less]
Abstract: Although pervious concrete was first used in the nineteenth century, it has only recently begun to increase in popularity. As urban areas expand, the problems associated with runoff management have become more challenging. The focus on the negative environmental effects associated with pavement runoff has also increased. These two issues have spurred the recent interest in pervious concrete pavements.Pervious concrete, however, has deficiencies which limit its application as pavements. These limitations include low compressive strength, flexural strength, clogging, and other durability issues. The overall purpose of this project was to provide tools to evaluate and improve the durability and strength of pervious concrete such that it may be more confidently employed in urban roadways. The specific objectives of this project were to (a) investigate the effect of mixture design on strength of pervious concrete (including the effect of fibers), (b) evaluate effect of clogging materials on coefficient of permeability, (c) and investigate the use of the dynamic pressurization test to evaluate the durability of pervious concrete, (d) develop a simple model for predicting removal of clogging particles from pervious concrete pavement surface pores. This thesis documents the results of the laboratory testing, and presents recommendations for mixture proportioning. In addition, recommendations are provided for optimizing the balance between compressive strength and permeability. URI: http://hdl.handle.net/1969.1/ETD-TAMU-2008-12-108 Files in this item: 1
Joung.pdf (1.085Mb) -
Stroud, Todd (May 2009)[more][less]
Abstract: Embryos derived by somatic cell nuclear transfer (SCNT) produce few pregnancies that result in a live, healthy offspring. This has largely been attributed to the aberrant reprogramming of the somatic cell DNA used for cloning. In order to improve the efficiency of cloning there is a great deal of research needed to determine the role of proteins involved in early embryonic reprogramming. In addition, studies are needed to determine effects on somatic and embryonic cell development as a result of altering these proteins. In this study we investigate the use of RNA interference in bovine somatic cells and embryos to knock down the expression of DNA methyltransferase-1 (DNMT1), an enzyme responsible for maintenance methylation in mammalian cells. We designed our experiments to test whether or not knocking down the DNMT1 gene would lead to a decrease in global methylation. It is our hypothesis that using somatic cells with reduced methylation may be advantageous for increasing the efficiency of cloning via somatic cell nuclear transfer. To accomplish this task, we have designed an infectious non-replicating lentiviral vector capable of delivering a gene that produces a short hairpin RNA targeting the mRNA of DNMT1. The construct included a sequence coding for green fluorescent protein (GFP) that will allow us to identify cells expressing the hairpin as well as a region coding for neomycin resistance so we could select for a pure population of transgenic cells to use for analysis. Infecting bovine fetal fibroblast cells with genes encoding shRNAs that target DNMT1 was successful. Quantitative real time PCR analysis of DNMT1 mRNA suggests that our shRNAs are capable of an 80% knockdown. The protein blot of indicates up to 90% knockdown of DNMT1. Cells transduced twice with a high titer virus showed the highest knockdown of both DNMT1 mRNA and the protein. Analysis of immunolabeled cytosine methylaiton showed a global decrease in DNA methylation as a result of the DNMT1 knockdown. However, double transduced cells with a high knockdown percentage of DNMT1 mRNA and protein became hypermethylated. The second experiment was conducted to determine the effect of injecting small interfering RNAs (siRNAs) targeting DNMT1 into oocytes prior to parthenogenic activation. This experiment was designed to give us information on the survivability and epigenetic profile of early embryos with decreased DNMT1. Oocytes injected with siRNA targeting DNMT1 had little development past the 8-cell stage as compared to the sham injected oocytes. This treatment group also had decreased DNA methylation as determined by immunolabeling of methylated cytosine residues. URI: http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-623 Files in this item: 1
Stroud.pdf (916.5Kb) -
Siu, Ho Kin (May 2008)[more][less]
Abstract: This thesis examines the connections between the motivations of ante rem structuralism and the problem of automorphism. Ante rem structuralists are led to the problem of automorphism because of their commitment to the thesis of structure-relative identity. Ladyman's and Button's solutions to the problem are both unsatisfactory. The problem can be solved only if ante rem structuralists drop the thesis of structure-relative identity. Besides blocking the problem of automorphism, there are further reasons why the thesis has to be dropped. (i) The purported metaphysical and epistemic purchase of adopting the thesis can be put into doubt. (ii) Primitive identity within a mathematical structure is more in line with ante rem structuralist's commitment to the faithfulness constraint and to the ontological priority of structure over positions. However, the cost of dropping the thesis is that ante rem structuralists cannot provide a satisfactory solution to Benacerraf's problem of multiple reductions of arithmetic. URI: http://hdl.handle.net/1969.1/ETD-TAMU-2008-05-14 Files in this item: 1
Siu_Thesis.pdf (325.9Kb) -
Bennett, Damon W. (August 2008)[more][less]
Abstract: This thesis presents the research results of a correlation and model based on nano and macroindentation hardness measurements. The materials used to develop and test the correlation include bulk tantalum and O1 tool steel. Following the literature review and a detailed description of the experimental techniques, the results of the nanoindentation hardness measurements are presented. After applying the methods and correlation recommended here, the results should give an accurate value of hardness in the Vickers scale for microstructural features that are too small to be precisely and exclusively measured using the traditional macroindentation hardness technique. The phenomena and influential factors in nanoindentation hardness testing are also discussed. These phenomena and theories are consistent with the microstructural behavior predicted in the Nix and Gao model for mechanism-based strain gradients. Implementing the correlation factors and/or correlation curve, accurate results can be found for metals over a broad hardness range. Initially, this research may impact the pipeline division of the petroleum industry by providing a correlation to the Vickers scale for nanoindentation testing of microstructural features. This thesis may also provide a research methodology to develop hardness correlations for materials other than metals. This thesis consists of eight chapters. Following an introduction in Chapter I, the research motivations and objectives are highlighted in Chapter II. Chapter III explains the multi-scale indentation techniques used in this thesis and Chapter IV presents the materials preparation techniques used. Then, the results are presented in Chapter V, followed by the factors affecting nanoindentation hardness in Chapter VI. Finally, Chapters VII and VIII reveal the indentation contact analysis, correlation, and conclusions of this research, respectively. URI: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-32 Files in this item: 1
Damon Bennett Thesis.pdf (10.64Mb)
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