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Real-time trafficking and assembly of ribosomal subunits

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dc.creator Wang, Jason en_US
dc.date.accessioned 2013-02-22T20:39:50Z
dc.date.available 2013-02-22T20:39:50Z
dc.date.created 2004 en_US
dc.date.issued 2013-02-22
dc.identifier.uri http://hdl.handle.net/1969.1/ETD-TAMU-2004-Fellows-Thesis-W26 en_US
dc.description Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. en_US
dc.description Includes bibliographical references (leaves 26-27). en_US
dc.description.abstract Nuclear pore complexes (NPCs) facilitate the bidirectional transport of a myriad of molecules, ranging in size from an ion to assembled ribosomal subunits, across the two lipid bilayers that constitute the nuclear envelope (NE). Research over the past decade has imparted much insight into how NPCs control the selective passage of large macromolecules while maintaining their compartmentalization properties. Schemes for soluble cofactor-mediated active transport have been developed through phenotypic mutational analyses and in vitro experiments. Continuing the effort for a more comprehensive characterization of NPC function, we have developed a method for the in vivo analysis of nucleocytoplasmic transport utilizing fluorescently labeled, recombinant human ribosomal protein L23a (rpL23a-His). Cytoplasmic microinjections into live HeLa cells indicated rapid nuclear import and subsequent export of rpL23a-His. These results are expected to lead to real-time in vivo single-molecule observation of ribosomal subunit trafficking and assembly. en_US
dc.format.medium electronic en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.publisher Texas A&M University en_US
dc.rights This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. en_US
dc.subject en_US
dc.subject Majors genetics and biochemistry. en_US
dc.title Real-time trafficking and assembly of ribosomal subunits en_US
thesis.degree.department en_US
thesis.degree.discipline en_US
thesis.degree.name Fellows Thesis en_US
thesis.degree.level Undergraduate en_US
dc.type.genre thesis en_US
dc.type.material text en_US
dc.format.digitalOrigin reformatted digital en_US

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