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Examination of dioxin and its alteration of gene expression via DNA Microarray Analysis

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dc.creator Wright, Justin Charles en_US
dc.date.accessioned 2013-02-22T20:40:16Z
dc.date.available 2013-02-22T20:40:16Z
dc.date.created 2004 en_US
dc.date.issued 2013-02-22
dc.identifier.uri http://hdl.handle.net/1969.1/ETD-TAMU-2004-Fellows-Thesis-W713 en_US
dc.description Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. en_US
dc.description Includes bibliographical references (leaves 18-26). en_US
dc.description.abstract Endocrine disruptive chemicals are known to produce harmful developmental effects on humans and other animals. Since substantial quantities of these chemicals are concentrated in the fat reserves of their victims, it is reasonable to expect a correlation between chemical concentration and physical effect before and to research further into the actuality of the EDCs effects. Some health hazards that are suspected to result from chemical exposure in humans are cleft lip and palate problems, feminization of male offspring, extreme premature puberty in female offspring, neural tube defects, autism, ototoxity, fetal alcohol syndrome (FAS), fetal tobacco syndrome (FTS), Type II diabetes also known as Non Insulin Dependent Diabetes Mellitus (NIDDM), and ADD/ADHD. Little evidence has been available to demonstrate how dioxin specifically alters gene expression, both in developing embryos and adults. Recently, Texas A&M University has acquired several DNA Microarray Systems, which are revolutionizing the examination of research into gene expression alterations. A new cell line of human embryonic kidney cells (293T/17 epithelial) have been properly cultured, had the RNA successfully isolated, and patterns were interpreted for genetic change using DNA Microarray Analysis. Through a cDNA slide specifically spotted with DNA of approximately 1200 endocrine regulated genes, the RNA of these cells can be examined using the DNA Microarray System after being exposed to different concentrations of dioxin, estradiol, DMSO, combinations of chemicals, and finally a control line of unexposed cells. Specific altered genes of the human embryo are predicted to be represented as changed in the RNA of cells previously exposed to dioxin. A nearly undetectable amount of dioxin (10⁴̄ M), was introduced to these cells, and produced significant variation from the natural gene expression. These data suggest that major advances in the prevention of physical pain and deformities in both developmental and everyday lives of both humans and animals could be attained by reducing exposure to environmental chemicals. The genes that are altered by the effects of TCDD promise further research, investigation, and prevention of many disruptive diseases. en_US
dc.format.medium electronic en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.publisher Texas A&M University en_US
dc.rights This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. en_US
dc.subject biomedical science. en_US
dc.subject Major biomedical science. en_US
dc.title Examination of dioxin and its alteration of gene expression via DNA Microarray Analysis en_US
thesis.degree.department biomedical science en_US
thesis.degree.discipline biomedical science en_US
thesis.degree.name Fellows Thesis en_US
thesis.degree.level Undergraduate en_US
dc.type.genre thesis en_US
dc.type.material text en_US
dc.format.digitalOrigin reformatted digital en_US


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